玻璃化
男科
胚泡
低温保存
活性氧
人类受精
化学
体外受精
胚胎发生
生物
胚胎
生物化学
解剖
细胞生物学
医学
作者
Mukesh Kumar Gupta,Sang Jun Uhm,Hoon Taek Lee
标识
DOI:10.1016/j.fertnstert.2010.01.043
摘要
To investigate the effect of vitrification and beta-mercaptoethanol (beta-ME) on reactive oxygen species (ROS) activity and in vitro development of oocytes vitrified before or after in vitro fertilization (IVF).Randomized prospective study.University-based assisted reproductive technology laboratory. ANIMALS(S): Abattoir-derived porcine ovaries.Oocytes were vitrified either before or 4 hours after the end of IVF by solid surface vitrification (SSV) without centrifugation and/or delipation procedure. beta-ME was used to inhibit ROS activity.Viability was evaluated by membrane integrity and esterase enzyme activity using fluorescein diacetate staining while ROS activity was assessed by 2',7'-dichlorofluorescein assay.Vitrification increased the ROS activity and decreased the viability and in vitro development of vitrified oocytes. Addition of beta-ME to vitrification and culture medium partially annihilated the ROS activity but did not improve the viability of vitrified-warmed oocytes. Furthermore, beta-ME had no effect on improving the fertilization ability of oocytes vitrified at metaphase II stage but significantly increased their ability to cleave. beta-ME also increased the rate of cleavage and blastocyst formation ability of oocytes vitrified 4 hours after the end IVF.Vitrification increases ROS activity in oocytes that can be partially annihilated by beta-ME to obtain enhanced embryonic development.
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