Simultaneous RP-HPLC estimation of Cinnarizine and Domperidone in Tablet

A simple reversed-phase high-performance liquid chromatographic method has been developed and validated for simultaneous estimation of Cinnarizine and Domperidone in tablets. The compounds were separated on a ‘Hypersil C18’ analytical column. The mobile phase used for separation was methanol: 0.05M phosphate buffer in the ratio (80:20 v/v). The pH was adjusted to 3.8 with ortho phosphoric acid. The flow rate was maintained to 1 ml/min. UV detection was performed at 275 nm. The method was validated for accuracy, precision, specificity, linearity, and sensitivity. Total chromatographic analysis time per sample was 10 min. The retention time for Cinnarizine and Domperidone was found to be 7.33 and 3.14 min respectively. Validation studies revealed that the method is specific, rapid, reliable, and reproducible. Calibration plots were linear over the concentration ranges of 10–35 μg/mL and 5–30 μg/mL for Cinnarizine and Domperidone respectively. The limit of detection and quantitation was 0.067 μg/ml and 0.221 μg/ml for Cinnarizine and 0.051 μg/ml and 0.169 μg/ml for Domperidone respectively. The high recovery and low relative standard deviation confirm the suitability of the method for determination of Cinnarizine and Domperidone in tablets.