类有机物
生物
神经科学
大脑
钙显像
人脑
胚胎干细胞
生物神经网络
神经网络
钙
中枢神经系统
基因
遗传学
内科学
医学
作者
Hidetsugu Sakaguchi,Yuki Ozaki,Tomoka Ashida,Takayoshi Matsubara,Naotaka Oishi,Sorin Kihara,Jun Takahashi
标识
DOI:10.1016/j.stemcr.2019.05.029
摘要
The cerebrum is a major center for brain function, and its activity is derived from the assembly of activated cells in neural networks. It is currently difficult to study complex human cerebral neuronal network activity. Here, using cerebral organoids, we report self-organized and complex human neural network activities that include synchronized and non-synchronized patterns. Self-organized neuronal network formation was observed following a dissociation culture of human embryonic stem cell-derived cerebral organoids. The spontaneous individual and synchronized activity of the network was measured via calcium imaging, and subsequent analysis enabled the examination of detailed cell activity patterns, providing simultaneous raster plots, cluster analyses, and cell distribution data. Finally, we demonstrated the feasibility of our system to assess drug-inducible dynamic changes of the network activity. The comprehensive functional analysis of human neuronal networks using this system may offer a powerful tool to access human brain function.
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