ENZYME SYSTEMS IN THE MYCOBACTERIA XV

Goldman, Dexter S. (Veterans Administration Hospital, Madison, Wis.). Enzyme systems in the mycobacteria. XV. Initial steps in the metabolism of glycerol. J. Bacteriol. 86: 30–37. 1963.—In cell-free extracts of strain H37Ra of Mycobacterium tuberculosis , glycerol is metabolized first by oxidation to dihydroxyacetone. A kinase was partially purified and shown to phosphorylate dihydroxyacetone; in the presence of the glyceraldehyde-3-phosphate dehydrogenase system, the product of the kinase reaction was further oxidized to 3-phosphoglycerate. The role of α-glycerol phosphate in the metabolism of strain H37Ra is discussed.