Enzyme–Acid Hydrolysis: An Effective Pretreatment Method for the Monosaccharide Composition Analysis of Acidic Polysaccharides

hydrolysis at optimized conditions (0.5-2.0 M, 60-90 min). This protocol increased the hydrolysis yields of PGP, PEP, and PFP to 97.41%, 86.17%, and 87.06%, respectively, with high repeatability and precision. These results were significantly higher than those from one-step hydrolysis methods, effectively improving the accuracy of the monosaccharide composition analysis for acidic polysaccharides.