Therapeutic effect of recuperating kidney and invigorating spleen prescription on rats with osteoporosis of spleen and kidney deficiency syndrome

Objective: Investigate deeply the therapeutic effect of recuperating kidney and invigorating spleen prescription on rats with spleen and kidney deficiency syndrome to provide experimental basis for the correlation study of spleen and kidney in the process of osteoporosis occurrence.Methods: Establish a rat osteoporosis model with spleen and kidney deficiency syndrome.On this basis,treat the model rats respectively with prescriptions of positive control,recuperating kidney,invigorating spleen,recuperating kidney and invigorating spleen.After three months administration,measure the indexes of TBV%,TRS%,TFS%,OSW,MAR and mAR,which can reflect the bone mass,bone formation and bone resorption.Results: Compared with the sham operation group,TBV% in spleen and kidney deficiency model group decreased significantly(P0.01),TRS%,TFS%,OSW,MAR and mAR increased significantly(P0.01).Compared with the model group,TBV% in all treatment groups increased obviously(P0.05,P0.01),TRS%,TFS%,OSW,MAR and mAR decreased obviously(P0.01).Compared with invigorating spleen group,TBV% increased and TRS%,TFS% decreased obviously in recuperating kidney group and recuperating kidney and invigorating spleen group(P0.05,P0.01),and the later reversed these indicators more significant(P0.05,P0.01).Conclusion: The methods of recuperating kidney,invigorating spleen,recuperating kidney and invigorating spleen have therapeutic effects on rats with spleen and kidney deficiency syndrome,and the methods of recuperating kidney and invigorating spleen work best.It points that in the process of osteoporosis,spleen and kidney of Chinese medicine are closely related.