Preparation of Polyclonal Antibody against Sulfonamide and Preliminary Study on ELISA Method

New Zealand White rabbits were immunized with three artificial immunizing antigens that modeling the common structure of sulfonamides,and the immune effect were detected by ELISA.A new Ci-ELISA method against sulfonamides and standard curves were established using two polyclonal antisera because of their good inhibitory effect.The IC10 of polyclonal antisera from group A against SMD,SMM,SMZ,SDM,SDM′ and SD was 0.010,0.046,0.010,0.067,0.019 μg/mL respectively,which was less than the MRLs(0.1 μg/mL).The IC10 of polyclonal antisera from group C against SMD,SMM,SMZ,SDM,SQ,SD,sulfachloropyridazine and sulfachaoropyrazine were 0.009 1,0.051,0.058,0.088,0.020,0.010,0.014,0.011 μg/mL respectively,less than the MRLs.The recoveries of two polyclonal antibodies ranged 54.5%-111.8%,54.2%-130.3% with CV of 3.6%-10.9% and 3.7%-5.9%,respectively.