医学
系膜细胞
肾病
转化生长因子
细胞生长
CTGF公司
下调和上调
肾小球硬化
作者
Min Wang,Di Yao,Suyu Wang,Qin Yan,Weiping Lu
出处
期刊:Endocrine
[Springer Nature]
日期:2016-04-15
卷期号:54 (1): 81-92
被引量:50
标识
DOI:10.1007/s12020-016-0950-5
摘要
Diabetic nephropathy as the primary cause of end-stage renal disease reveals an increased incidence in patients with kidney disease as the continuous rising of type 2 diabetes. Long non-coding RNAs (lncRNAs) are involved in the development of many diseases including diabetes; however, the role of lncRNAs in diabetic nephropathy is still unclear. In the present study, lncRNA microarray analysis was used to identify abnormally expressed lncRNAs and nearby mRNAs in renal cortical tissues dissected from kidney of db/db and db/m mice. After verifying the data from microarray analysis by quantitative RT-PCR, downregulated ENSMUS0147869 associated with Cyp4a12a was selected for overexpression in mouse mesangial cells among differentially expressed lncRNAs. Cell Counting Kit-8, Western blotting, and quantitative RT-PCR showed that proliferation and fibrosis indexes were reversed in mesangial cells with ENSMUS0147869 overexpression. Our data suggested the potential role of ENSMUS0147869 in proliferation and fibrosis of mesangial cells, which provided a molecular biomarker and therapeutic target for diabetic nephropathy.
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