HPLC determination of enantiomer in buagafuran

Objective:To establish an HPLC method for dermination of enantiomer as impurities in buagafuran. Methods:The Daicel Chiralcel OD column was used with n - hexane as mobile phase,flow rate was 0.5 mL·min~(-1),column temperature was 30℃,injection volume was 10μL and wavelength was 196 nm.Results:Under this chromatographic condition,the resolution of buagafuran and its enantiomer was 4.24.Enantiomer showed a good linearity in the range of 1 - 20μg·mL~(-1)(r = 0.9999).The limits of detection and quantitation of the enantiomer were 3 ng and 10 ng,respectively.The average recovery and RSD were 101.0%and 1.4%(n =9).There was no enantiomer being detected in buagafuran.Conclusions:The method is rapid,simple and can be used for separation and determination of enantiomer in buagafuran.