Autoantibodies detection in autoimmune liver disease patients

Objective To detect the autoantibodies in autoimmune liver disease patients and analyse the positive ratio. Method Detection of a group of autoantibodies in 178 autoimmune liver disease patients and 200 normal adults using indirect immunofluorescennce assay and immunoblot assay. Patients divided into 3 groups: AIH, PBC and unknown cause liver injury. Analysing the positive ratio of autoantibodies in each group. Results The AIH group can divide into 3 subtypes according to the difference of the autoantibodies as Ⅰ(75%), Ⅱ(8.3%), Ⅲ(16.7%). In the PBC group, the patients are all AMA positive, the positive rate of M2 is 95.5%. The autoantibodies appear in 80.0% unknown cause liver injury group in which 22 patients found none autoantibodies and 5 cases presented unknown autoantibodies. Conclusion Autoimmune liver disease is progressive inflammation of the liver accompanied with the production of characteristic antibodies. Detection of autoantibodies are useful clinically for identifying and classifying liver disease.