Study on antioxidant activity of polysaccharides from Pleurotus.Nebrodensis,Pleurotus.eryngii and Pleurotus.ferulae

The polysaccharides of Pleurotus.Nebrodensi,Pleurotus.eryngii and Pleurotus.ferulae fruit body were extracted with hot water extraction and ethanol precipitation method.Antioxidant activity of the polysaccharides from Pleurotus.Nebrodensis、Pleurotus.eryngii and Pleurotus.ferulae fruit body was studied by several experiments,including the salicylic acid method,the self oxidation method of pyrogallol,reducing power measure and the method of eliminating of NO-2 in mimic gastric juice.The results showed the polysaccharides extracted from Pleurotus.Nebrodensis,Pleurotus.eryngii and Pleurotus.ferulae fruit body all had strong antioxidant properties in vito.And with the concentration of polysaccharides increasing,its antioxidant activity gradually become strong.For the capacity of scavenging hydroxyl radical(·OH) by three different polysaccharides,Pleurotus.Nebrodensis showed the strongest,followed by Pleurotus.eryngii and Pleurotus.ferulae the weakest.In the experiment of scavenging superoxide anion radical(O-2·),Pleurotus.eryngii had the strongest power,Pleurotus.Nebrodensis the second and Pleurotus.ferulae was the weakest.In the reducing power measure and eliminating of NO-2,Pleurotus nebrodensis showed the strongest,followed by Pleurotus eryngii,Pleurotus.ferulae were in the worst performance.