Twelve wild mulberry varieties are crossed with 6 cultivars of mulberry. Thirty two distant combinations of differ ploidys are obtained. Among them, the seed formation and survival rate of seedlings of hybrids of wild diploids × diploid cultivars are higher than that of hybrids of wild hexaploids ×diploid cultivars; and hybrids of diploid cultivars × wild diploids are higher than that of hybrids of diploid cultivars × wild hexaploids ; and hybrids of cultivars × wild mulberry are higher than that of hybrids of wild mulberry × cultivars.