Research on the Conditions for Laccase Production from Trametes versicolor and Effect of Heat Shock on Laccas

[Objective] The study aimed to provide an effective control strategy for enhanceing the level of laccase fermentation.[Method] With strain of trametes versicolor 14001,conditions for laccase production and the effects of heat-shock activity on fermentation was studied.[Result] The results indicated that the optimal fermentation conditions were as follows:Cu2+ concentration 20 mg/L,initial pH4.5 of the medium,inoculum percentage 10% and at 25 ℃.Enzyme activity improved 30% compared with not pH cortrol.Heat shock could induce the corresponding the mRNA of laccase,increased the laccase by one times.[Conclusion]The optimized performance and heat shock could enhance fermentation leverl of laccase.