The role ofTrichoderma harzianumin regulating soil enzymatic activities and soil microbial community were studied by using a pot seeding experiment.The results showed thatT.harzianumcould effectively regulated soil microbial community in rhizosphere soil during rice seedling stage,however,it produced different effects on bacteria, fungi and actinomycete.In the 28th day after sowing,the amount of bacteria and actinomycete under inoculation treatmentwas increased by 50.70%and 48.56%,respectively,butthe amountof fungi was reduced by 16.15%,compared with those under control;moreover,the amount ofT.harzianumunder inoculation treatmentwas 138.46%more than that in the sowing day.T.harzianumcould also improve the activitiesof urease,phosphatase and invertase,whichwere increased by 8.55%,18.31%and 49.61%,respectively,under inoculation treatment.These results showed thatT. harzianumhad the ability to improve soil micro-ecological environment.