Comparative Study on 4 Kinds of Extraction Methods of Genomic DNA from Fusarium spp.

[Objective] The aim was to search a convenient and high efficient extraction method for genomic DNA to provide the scientific basis for further developing the molecular biological study of filamentous fungi such as Fusarium spp.[Method] The genomic DNA from 8 Fusarium spp.strains were extracted by 4 methods of improved CTAB,SDS,high salt precipitation and SDS-CTAB and the effects of the different extraction methods were compared through the quality detection and PCR analysis.[Result] The 4 extraction methods all could extract the genomic DNA and their order of the extraction quality from good to bad was SDS,SDS-CTAB,high salt precipitation and CTAB.Among them,the SDS method could extract the genomic DNA of all the tested strains successfully and the concn.and the purity of the DNA were all higher,the pollution by RNA and other impurity was little.While the genomic DNA extracted by high salt precipitation and CTAB were not integrity or its concn.was lower.[Conclusion] The SDS method was more suitable for the extraction of genomic DNA from filamentous fungi such as Fusarium spp..