Cordycepin,one of the important compounds in Cordyceps militaris,has the activities of anti-tumor,immune regulation,antimicrobial,anti-inflammatory,and so on.Cordycepin is extracted from cultured Cordyceps militaris.The yield of cordycepin is improved by strain breeding in the optimized culture medium.In this paper,bioactivity analysis,production enhancement and determination methods of cordycepin have been discussed.The further research directions of cordycepin have also been proposed.