Vasomotor Effect of Salidroside on Rat Mesenteric Artery and Its Regulatory Mechanisms

Objective: To observe vasomotor effect of salidroside( SAL) on rat mesenteric artery,and to explore its underlying regulatory mechanisms. Method: Tension was measured by DMT system to evaluate the vasomotor effect of SAL on rat endothelium-intact and endothelium-denuded mesenteric aortic rings. Different antagonists and ELISA method were used to illustrate the mechanisms of vasomotor effect of SAL. Result: 1 SAL had significantly vasoconstrictive effect on mesenteric aortic rings precontracted by KCl. 2 SAL relaxed endothelium-intact and endothelium-denuded mesenteric artery precontracted by PE in concentration-dependent manner,while the vasodilation of endothelium-intact mesenteric artery was significantly stronger than that of endothelium-denuded one. 3L-NAME attenuated the vasodilation of endothelium-intact mesenteric artery induced by SAL,while neither indomethacin nor methylene blue affected it. 4 Using ELISA,SAL can significantly increase NO and NOS of mesenteric artery. Conclusion: The results indicate that SAL plays a dual role of vasoconstriction and vasodilation in regulating mesenteric artery. Vasoconstriction may be associated with voltage-dependent calcium channel,while vasodilation may be associated with inhibition of intracellular calcium release. Besides,vasodilation shows endothelium dependence,which is related to NO released and NOS synthesized,regardless of the COX pathway.