Sudhausia floridensis n. sp. (Nematoda: Diplogastridae) isolated from Onthophagus tuberculifrons (Coleoptera: Scarabaeidae) from Florida, USA

Sudhausia floridensis n. sp., a phoretic associate of a dung beetle, Onthophagus tuberculifrons , is described based on its typological characters and molecular profiles of part of the small subunit ribosomal RNA (rRNA) gene, D2-D3 expansion segments of the large subunit of rRNA gene, as well as part of the mitochondrial cytochrome oxidase subunit I ( mtCOI ) gene. Adult beetles collected from the cattle unit at the University of Florida, Gainesville, FL, USA were dissected and placed on a water agar plate to allow nematode propagation. The new species was isolated from a culture plate several weeks later, and subcultured to establish a laboratory strain. Sudhausia floridensis n. sp. is typologically very similar to S. aristotokia , but could be distinguished from it by the shape of the tail tip of the male, and presence vs absence of a terminal notch in the bursal velum. Molecularly, S. floridensis n. sp. is close to S. crassa , regardless of its morphological similarity to S. aristotokia , but it is distinguished from its African congeners S. aristotokia and S. crassa by 28 and 12 bp differences in SSU, respectively.