면역학적 거부 반응을 관찰할 수 있는 새로운 폐 이식술 : 피하 폐 이식술

Purpese: Lung transplantation is now accepted as an effective therapy for end-stage pulmonary vascular and parenchymal diseases. Rejection is a major impediment to long-term survival of lung transplant recipients. Lung allograft rejection has been studied in various animal models. To study the immunological mechanism of its rejection the studies on lung allograft rejection must be performed in inbred animals such as mice or rats, However, it is very delicate and difficult to transplant the lung in small inbred animals, especially in mice. The technical difficulty hampered the investigations of lung allograft rejection. Methods: This study introduced the new lung transplantation technique in mice for immunological study, subcutaneous lung tissue transplantation, in which the piece of lung tissue with 1-1.5 mm thickness was introduced subcutaneously through incision site on flank and transplanted to subcutaneous site of shoulder of mouse. Histological changes were followed up in transplanted lung tissues for 18 to 21 days. Lung tissues from CBA mice or Balb/c mice were subcutaneously transplanted to Balb/c mice in experimental or control group respectively. Results: Histological changes in the grafts of experimental groups could be divided into 4 phases, inflammatory, immunological, necrotic and fibrotic phase, Immunological or necrotic phase in this study correlated with grade 1-3 or grade 3-4 of acute lung rejection classified by the Lung Rejection Study Group. Conclusion: It is concluded that the subcutaneous lung tissue transplantation can be a technique for immunological study on acute lung allograft rejection in mice.