Sensitive Immunoassay Based on Biocompatible and Robust Silica-Coated Cd-Free InP-Based Quantum Dots

High Resolution Image Download MS PowerPoint Slide Low-toxic InP quantum dots (QDs) as an ideal candidate for Cd-based QDs have tremendous potential for next-generation commercial display and biological detection applications. However, the progress in biological detection is still far behind that of the Cd-based QDs. This is mainly because the InP-based QDs are of inferior stability and photoluminescence quantum yield (PL QY) in aqueous solution. Here, PL QY of 65% and excellent stability of InP/GaP/ZnS [email protected] 2 nanoparticles have been successfully synthesized via a silica coating method. The containing thiol-capped hydrophobic InP/GaP/ZnS QDs were pre-silanized with waterless, ammonia-free hydrolysis tetraethyl orthosilicate, and subsequently, an outer silica shell was generated in the reverse microemulsion. The corresponding QD-based fluorescence-linked immunosorbent assay exhibits a high sensitivity of 0.9 ng mL –1 for C-reactive protein and the broad detection range of 1–1000 ng mL –1, which was close to that of the state-of-the-art Cd-based [email protected] 2 nanoparticles and had the highest sensitivity of Cd-free QDs so far. This work provides a very successful silica coating method for the containing thiol-capped hydrophobic QDs and the QDs highly sensitive to water and oxygen, and the obtained InP/GaP/ZnS [email protected] 2 nanoparticles were considered as the robust, biocompatible, and promising Cd-free fluorescent labels for the further ultra-sensitive detection.