生物
巨噬细胞
细胞内
细胞内寄生虫
人口
微生物学
单核吞噬细胞系统
单核细胞
外周血单个核细胞
脾脏
免疫系统
表型
沙门氏菌
病菌
免疫学
病毒学
细菌
细胞生物学
体外
基因
遗传学
社会学
人口学
作者
Dotan Hoffman,Yaara Tevet,Sébastien Trzebanski,Gili Rosenberg,Leia Vainman,Aryeh Solomon,Shelly Hen-Avivi,Noa Bossel Ben-Moshe,Roi Avraham
出处
期刊:Immunity
[Elsevier]
日期:2021-12-01
卷期号:54 (12): 2712-2723.e6
被引量:24
标识
DOI:10.1016/j.immuni.2021.10.015
摘要
Interactions between intracellular bacteria and mononuclear phagocytes give rise to diverse cellular phenotypes that may determine the outcome of infection. Recent advances in single-cell RNA sequencing (scRNA-seq) have identified multiple subsets within the mononuclear population, but implications to their function during infection are limited. Here, we surveyed the mononuclear niche of intracellular Salmonella Typhimurium (S.Tm) during early systemic infection in mice. We described eclipse-like growth kinetics in the spleen, with a first phase of bacterial control mediated by tissue-resident red-pulp macrophages. A second phase involved extensive bacterial replication within a macrophage population characterized by CD9 expression. We demonstrated that CD9+ macrophages induced pathways for detoxificating oxidized lipids, that may be utilized by intracellular S.Tm. We established that CD9+ macrophages originated from non-classical monocytes (NCM), and NCM-depleted mice were more resistant to S.Tm infection. Our study defines macrophage subset-specific host-pathogen interactions that determine early infection dynamics and infection outcome of the entire organism.
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