倍半萜
自噬
细胞凋亡
倍半萜内酯
化学
生物发生
细胞毒性T细胞
癌细胞系
传统医学
癌细胞
体外
癌症
生物化学
立体化学
生物
医学
基因
遗传学
作者
Bao-Jia Yang,Juan Wang,Zheng-Quan Zeng,Yang Xu,Ai-Ying Huang,Xiao‐Jiang Hao,Xiao Ding,Shun‐Lin Li
标识
DOI:10.1080/14786419.2021.1955881
摘要
Fourteen sesquiterpene lactones were isolated from the whole plant of Carpesium abrotanoides L. Their structures were determined on the basis of comprehensive spectroscopic data analysis. All compounds were screened for their cytotoxic activity, and compound 6 showed the strongest activity (IC50 2.73 - 7.21 µM) against five human cancer cell lines, including A549, HepG2, HCT116, MDA-MB-231, and CNE2. Compound 6 was further investigated. Compound 6 effectively induced G2/M cell cycle arrest and ROS accumulation in a dose-dependent manner, which further led to apoptosis in cancer cells. Interestingly, compounds 1 and 6 could also activate protective autophagy, which was reported for the first time in sesquiterpene isolated from Carpesium abrotanoides. In addition, compounds 1 and 6 could induce lysosomal biogenesis by 173.2% and 163.7%, respectively. In sum, sesquiterpene lactones from Carpesium abrotanoides could induce apoptosis and protective autophagy in cancer cells, which provide a serial of compounds with potential clinical applications.
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