甲基化
生物
RNA甲基化
DNA甲基化
核糖核酸
基因表达调控
基因
转录组
基因表达
遗传学
甲基转移酶
作者
Mengqi Chang,Hongyi Lv,Weilong Zhang,Chunhui Ma,Xue He,Shunli Zhao,Zhi-wei Zhang,Yi‐Xin Zeng,Shuhui Song,Yamei Niu,Wei‐Min Tong
出处
期刊:Open Biology
[The Royal Society]
日期:2017-09-01
卷期号:7 (9)
被引量:151
摘要
N 6 -methyladenosine (m 6 A) is the most abundant epitranscriptomic mark found on mRNA and has important roles in various physiological processes. Despite the relatively high m 6 A levels in the brain, its potential functions in the brain remain largely unexplored. We performed a transcriptome-wide methylation analysis using the mouse brain to depict its region-specific methylation profile. RNA methylation levels in mouse cerebellum are generally higher than those in the cerebral cortex. Heterogeneity of RNA methylation exists across different brain regions and different types of neural cells including the mRNAs to be methylated, their methylation levels and methylation site selection. Common and region-specific methylation have different preferences for methylation site selection and thereby different impacts on their biological functions. In addition, high methylation levels of fragile X mental retardation protein (FMRP) target mRNAs suggest that m 6 A methylation is likely to be used for selective recognition of target mRNAs by FMRP in the synapse. Overall, we provide a region-specific map of RNA m 6 A methylation and characterize the distinct features of specific and common methylation in mouse cerebellum and cerebral cortex. Our results imply that RNA m 6 A methylation is a newly identified element in the region-specific gene regulatory network in the mouse brain.
科研通智能强力驱动
Strongly Powered by AbleSci AI