Gas Chromatography-mass Spectrometry Coupled with Solid-phase Microextraction for Determination of Volatile Constituents of Chinese Ginger

Gas chromatography-mass spectrometry combined with solid-phase microextraction (SPME-GC-MS) was developed for the determination of volatile compounds of ginger. The volatile constituents of fresh ginger were extracted developed by SPME with a 100 um polydimethylsiloxane fiber. The fibers were desorbed in a GC injection liner at 250oC for 3 min. With headspace SPME-GC-MS analysis, thirty-six peaks in ginger were separated by mass spectrometry. The volatile compounds included α-phellandrene, camphene, linalool, geranial, zingberene, sesquiphel-landrence, neral, α-bisabolene, α-curcumene etc and their relative contents were calculated on basis of peak area ratio. SPME extraction conditions including time, temperature and desorption time were investigated.