Enhancer and Gene Trap Transposon Mutagenesis in Arabidopsis

Insertional mutagenesis in Arabidopsis can be conveniently accomplished either by Agrobacterium mediated TDNA transformation, or by transposable element mobilization. Recent advances have made T-DNA transformation one of the most efficient methods to generate large numbers of insertions in the Arabidopsis genome (1-3). Typically, several hundred independent T-DNA insertions can be generated per transformed plant. This is many more insertions than in most transposon schemes, but despite the labor involved, transposons have some important advantages: