Effects of Artocarpus Lingnanensis Lectin on Proliferation of Jurkat T Lymphocytes

Objective To study Artocarpus lingnanensis lectin(ALL)on the growth of acute lymphoblastic leukemia Jurkat T lymphocytes in vitro.Methods Jurkat cells were incubated with different concentrations of ALL.The proliferation of Jurkat T lymphocytes was analyzed by using WST-8 colorimetric assay and inverted Microscope.The level of IL-2,IL-10 and IFN-γ in the supernatants was measured by enzyme linked immune sorbent assay(ELISA).Results ALL concentration in the range of 0.36-360 μg/ml inhibited the proliferation of Jurkat cells,showing dose-response relationship.Inhibition rates were in the range of 1.82%-59.17%(P0.01),and its IC50 was 59.72 μg/ml.It was also observed that the colony formation of Jurkat T cells decreased along with the increase of ALL concentration.The secretion of IL-2 and IFN-γ by Jurkat cells were enhanced.Compared with the control group,the level of IL-2 and IFN-γ were 11.69 times and 21.29 times higher respectively in ALL(1.8 μg/ml)treatment group(P0.01).Conclusion ALL can suppress Jurkat T lymphocytes proliferation,showing a dose effect relationship.