Improvement of methods for extracting high quality total RNA from sheep adipose tissue and its usage

An improved method of extracting the total RNA from ventral subcutaneous adipose tissue,mesentery adipose tissue and tailhead adipose tissue of sheep was developed and the expression and tissue distribution of the fatty acid synthase(FAS)gene in sheep were investigated.The result showed that:total RNA extracted from adipose tissue by the improved method was pure,integral,high quality and without chromosome DNA contamination.The expression of FAS mRNA in the ventral subcutaneous adipose tissue were significantly different compared with tailhead adipose tissue and mesentery adipose tissue(P0.01),but there was no significant difference between tailhead adipose tissue and mesentery adipose tissue(P0.05).