Objective Through to infects the pathogen to scove points the meeting and parting examination ,Aehieved in the prevention and the control courtyard infects goal .Methods According to the different sources of the pathogen of the cause ofinfection ,Using different examining methods .Results In the pathogen separated from tfe infective parts , G-bacterium takes up 56.5% ,G+bacterium 34.4% ,fungus and other bacteria 9.3% ,Which fits the tend of the developing of pathogen ,That is G- bacterium is adding ,While G+ bacterium is reducing .Conclusion Strengthening the test and examination of the microbe is very useful to prevent and control the infection around the hospitai .