Study on the content measurement and quality evaluation of ginsenoside constituent from different habitats with UPLC analysis method

Objective: To study the content difference and quality evaluation of ginsenoside constituent from different habitats with ultra-high performance liquid chromatography(UPLC). Methods: 20 batches of ginseng samples originated in Liaoning, Jilin and Heilongjiang provinces were collected, where the contents of 9 kinds of ginsenoside constituent(Rg1, Re, Rb1, Rf, Rg2, Rc, Rb3, Rb2, Rd) were measured with UPLC. Results: The average contents of 9 kinds of ginsenoside constituent in Liaoning and Jilin provinces were significantly higher than those in Heilongjiang province. The results of content sequence of ginsenoside showed that the top 15 samples were all originated in Jilin and Liaoning provinces. 3 kinds of ginsenosides(Rg1, Re, Rb1) analysis results showed that the contents of ginsenosides had obvious differences in different provinces. The contents of ginsenosides Rg1, Re and Rb1 in Qingyuan of Liaoning province, Fusong of Jilin province and Changbai of Jilin province were higher than those in other habitats. The similarities in the chromatographic fingerprints of 20 batches samples were all above 0.93. Conclusion: The highest contents of ginsenosides in ginseng samples originates in Jilin province, as followed Liaoning province and Helongjiang province. The results show that The quality of ginseng is stable with consistent chemical component.