Objective To study the collective regulations of catalpol in different parts of Rehmannia glutinosa Libosch.Methods HPLC method was used the Mobile phase was cetonitrile-water(0.5∶99.5)and detection wavelength was 210 nm.Results The standard curve of catalpol was Y=347.93X + 10.138(r=(0.999 6))with the linear range of 0.322 4~2.901 6 μg,the average recovery was 96.21%(n=5).Conclusion The method is simple,sensitive and rapid,and collective regulations of catalpol in different parts of Rehmannia glutinosa Libosch are apparent.