Electroporation is the use of short, high-voltage pulses to transiently permeabilize the cell membrane. This technique can be used to increase the cellular uptake of otherwise non-permeating molecules, e.g., cytotoxic agents (electrochemotherapy). The electrochemotherapy area can also be used as a tumorlysis product in connection with immunotherapy. New avenues are opening for the clinical use of electroporation in non-viral gene delivery.