Biodegradation of PAHs by <i>Trametes hirsuta</i> zlh237 and Effect of Bioaugmentation on PAHs-Contaminated Soil

This study aimed to investigate the biodegradation of three distinct PAHs: Phenanthrene (Phe), Pyrene (Pyr), and Benzo[a]pyrene (BaP) by using fermentation broth from T. hirsuta culture medium.The results revealed that 2,5-xylidine ameliorates laccase production in T. hirsuta fermentation medium.After 6 days of incubation, T. hirsuta zlh237 fermentation broth (6-day-FFB) showed a higher degradation rate for three PAHs than 11-day fermentation broth (11-day-FFB).Final Phe, Pyr, and BaP degradation rates of 6-day-FFB were 89.35%, 85.92%, and 89.45%, respectively, on the 5 th day of incubation.The PAHs biodegradation rate of 6-day-FFB in natural soil samples were lower than in sterilized soil.The Phe, Pyr, and BaP degradation rates of 6-day-FFB were 54.49%, 46.76%, and 51.93% in sterilized soil samples, and 39.37%, 34.00%, 33.99% in natural soil samples, after 15 days incubation, respectively.The high-throughput sequencing analysis revealed that the 6-day-FFB altered the bacterial community structure and enhanced microbial biodiversity of contaminated soil.The PAHs-contaminated soils incubated with 6-day-FFB showed significant increase in well color development than the controls.Principal Component Analysis of Biolog data differentiated the effect of 6-day-FFB and sterilized 6-day-FFB on contaminated soils.It implied that T. hirsuta zlh237 restored the microbiological functioning of the PAHs contaminated soils.