光遗传学
转化生长因子
细胞信号
信号转导
细胞
神经科学
细胞生物学
生物
遗传学
出处
期刊:Methods in molecular biology
日期:2022-01-01
卷期号:: 113-124
被引量:1
标识
DOI:10.1007/978-1-0716-2277-3_9
摘要
Cells employ signaling pathways to make decisions in response to changes in their immediate environment. The Transforming Growth Factor β (TGF-β) signaling pathway plays pivotal roles in regulating many cellular processes, including cell proliferation, differentiation, and migrations. In order to manipulate and explore the dynamic behavior of TGF-β signaling at high spatiotemporal resolution, we developed an optogenetic system (the optoTGFBRs system), in which light is used to control TGF-β signaling precisely in time and space. Here, we describe about experimental details of how to build the optoTGFBRs system and utilize it to manipulate TGF-β signaling in a single cell or a cell population using microscope or LED array, respectively.
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