PI3K/AKT/mTOR通路
蛋白激酶B
细胞凋亡
生物
标记法
卵泡发生
卵泡液
LY294002型
卵泡期
流式细胞术
内分泌学
内科学
癌症研究
细胞生物学
信号转导
男科
免疫学
卵母细胞
医学
生物化学
胚胎
低温保存
作者
Ying Han,Ruqiang Yao,Zexin Yang,Shuang Li,Meng Wei,Yinfeng Zhang,Yunshan Zhang,Haining Luo
标识
DOI:10.1016/j.yexcr.2021.113002
摘要
The inflammatory microenvironment has been demonstrated to play a role in folliculogenesis, ovulation and premature ovarian failure (POF), as well as infertility. In this study, we aimed to explore the role of inflammation in modulating growth and apoptosis in granulosa cells (GCs), the main components of ovarian follicles. ELISA was used to analyze the levels of inflammatory factors (IL-1β, IL-4, IL-6 and IL-10) in follicular fluid samples and GCs derived from POF patients and healthy normal individuals. CCK-8, flow cytometry and TUNEL assays were used to assess the effect of IL-4 on GC growth and apoptosis. Western blotting was used to examine the effect of IL-4 on the activation of PI3K/Akt, Erk1/2 and Jnk signaling. The results showed that IL-4, IL-1β and IL-6 levels were increased in follicular fluid samples and GCs derived from POF patients compared with those from healthy individuals. GC growth was weakened when cells were treated with IL-4, while apoptosis was increased. In addition, IL-4 increased the level of p-Akt/Akt in GCs. In addition, LY294002, an inhibitor of PI3K, abolished the effect of IL-4 by inhibiting GC growth and promoting apoptosis. In summary, this study demonstrated that IL-4 levels were increased in POF samples and that IL-4 could inhibit GC growth and induce GC apoptosis by activating PI3K/Akt signaling.
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