Selecting the right elution buffer for mRNA purification using the POROS Oligo (dT)25 Affinity Resin

The POROS™ Oligo (dT)25 Affinity Resin helps to address the selectivity and capacity requirements for the large-scale manufacturing of mRNA used in vaccine and gene therapy applications. Typically, mRNA binds to the Oligo (dT)25 affinity resin using high ionic strength conditions and neutral pH and is eluted from the column using low ionic strength solutions such as water. Although water works well for most mRNA constructs, a need to identify alternative elution buffers to optimize mRNA recovery exists. This article describes experiments conducted to optimize mRNA purification using high-throughput screening.