Thiazole- and Coumarin-Conjugated (β-Lactam Scaffold) Azetidinones Synthesis and Their Substitution Effect in In Silico, and In Vitro Cell Viability Studies

In present pandemic situations, we need more and more care on the medication, to treat the ill cells or to control the growth of the cells we need better transfection with active pharmaceutical ingredients. In chemotherapy many drugs are working on the target as well as on adjacent organs, to avoid such conditions, we required active compounds, coumarin, and thiazole-based compounds can function in this way. This research work illustrated better transfection and percentage of cell viability of azetidinones by the insertion of thiazole and (or) coumarin groups. Synthesized the tilted compounds from Thiazole (or) Coumarin-based Schiff base, and chloroacetyl chloride with good yields. Herein, the β-lactam group acts as a linker, to this linker one side heterocyclic ring acts as the anchoring group, and the other side of the aromatic ring acts as the hydrophobic group; in silico studies of this series, results have shown that many compounds are having anti-cancer activity along with other biological activities. The in vitro cell viability studies IC50 values of Azetidinone (III): 1.5608, (IV): 1.5694, (V): 1.5489, and (VI): 1.7452 are better than the reference Lipofectamine 2000 (L2K): 2.0280 against oral cancer cell line (CAL-27), this result supported by the cellular uptake against HEK-293, DNA binding, and β-galactose activity.