A sensitive miRNA detection method based on a split-T7 switch modulating CRISPR/Cas12a system

This study presents a novel method for sensitive miRNA detection based on a split-T7 switch modulating CRISPR/Cas12a system. By integrating the split-T7 promoter-mediated transcription with the CRISPR/Cas12a system, this method can achieve femtomolar detection of the target miRNA within 1 h and successfully analyze miR-21 in samples from various cell lines, demonstrating its potential for clinical applications.