Isolation and Structural Elucidation of Stigmasterol, β-Sitosterol, and Tripalmitolein from the Leaf of Vitex Chrysocarpa (Lamiaceae)

Vitex chrysocarpa (Lamiaceae) is used in traditional medicine to treat fever, dysmenorrhea, venereal diseases, and dysentery. However, more research needs to be done on the compounds responsible for these activities. This study aims to isolate and elucidate the main bioactive compounds from the plant’s methanol leaf extract. The maceration method was utilized for the extraction, while column and thin-layer chromatographic techniques were employed for purification and isolation. As a result, Tripalmitolein (4.7 mg) and a combination of Stigmasterol and β-sitosterol (7 mg) were isolated. Careful examination of the 1D and 2D NMR data allowed for the structural elucidation of the compounds, and additional confirmation of the compounds were obtained by comparing the results with those of the existing literature. This study presents the first isolation and structural elucidation of Stigmasterol, β-sitosterol, and tripalmitolein from Vitex chrysocarpa.