脱落酸
内生
褪黑素
多年生植物
叶绿体
转录组
转基因作物
转基因
活性氧
光合作用
生物
细胞生物学
生物合成
生物化学
植物
化学
基因表达
基因
神经科学
作者
Chao Li,Tengteng Gao,Danni Zhang,Shengming Xu,Xin Jia,Xiaomin Liu,Kexin Tan,Yi Zhou,Zhijun Zhang,Fengwang Ma
出处
期刊:Authorea - Authorea
日期:2023-09-04
标识
DOI:10.22541/au.169384151.12357170/v1
摘要
High temperature negatively impacts the yield and quality of fruit crops. Exogenous melatonin (MT) application has shown the capability to enhance heat tolerance, but the response of endogenous MT to heat stress, particularly in perennial fruit trees, remains elusive. This study investigated the effects of high temperatures on transgenic apple plants overexpressing the MT biosynthetic gene N-acetylserotonin methyltransferase 9 ( MdASMT9 ). Endogenous MT protected transgenic plants from heat stress, scavenging reactive oxygen species (ROS) and increasing soluble carbohydrates and amino acids levels. MdASMT9 -overexpressing plants also maintained higher photosynthetic activity by protecting the chloroplasts from damage. Transcriptome sequencing indicates that MdASMT9 overexpression promoting the expression of HSFA1d , HSFA2-like , and HSFA9b , and inhibiting the transcription of HSFB1 and HSFB2b . Application of MT and overexpression of MdASMT9 reduced abscisic acid (ABA) accumulation through promoting MdWRKY33-mediated transcriptional inhibition of MdNCED1 and MdNCED3 , thus promoting stomatal opening for better heat dissipation. Furthermore, melatonin enhanced autophagic activity through promoting MdWRKY33-mediated transcriptional enhancement of MdATG18a under heat stress . These findings provide new sight into the regulation of endogenous MT and its role in improving heat tolerance in perennial fruit trees.
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