材料科学
导电体
诱导多能干细胞
导电聚合物
脚手架
生物医学工程
组织工程
纳米技术
聚合物
胚胎干细胞
化学
复合材料
医学
基因
生物化学
作者
Gisselle Gonzalez,Aileena C. Nelson,Alyssa R Holman,Alexander J. Whitehead,Erin LaMontagne,Rachel Lian,Ritwik Vatsyayan,Shadi A. Dayeh,Adam J. Engler
出处
期刊:Biomaterials
[Elsevier]
日期:2023-11-01
卷期号:302: 122363-122363
标识
DOI:10.1016/j.biomaterials.2023.122363
摘要
Despite numerous efforts to generate mature human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs), cells often remain immature, electrically isolated, and may not reflect adult biology. Conductive polymers are attractive candidates to facilitate electrical communication between hPSC-CMs, especially at sub-confluent cell densities or diseased cells lacking cell-cell junctions. Here we electrospun conductive polymers to create a conductive fiber mesh and assess if electrical signal propagation is improved in hPSC-CMs seeded on the mesh network. Matrix characterization indicated fiber structure remained stable over weeks in buffer, scaffold stiffness remained near in vivo cardiac stiffness, and electrical conductivity scaled with conductive polymer concentration. Cells remained adherent and viable on the scaffolds for at least 5 days. Transcriptomic profiling of hPSC-CMs cultured on conductive substrates for 3 days showed upregulation of cardiac and muscle-related genes versus non-conductive fibers. Structural proteins were more organized and calcium handling was improved on conductive substrates, even at sub-confluent cell densities; prolonged culture on conductive scaffolds improved membrane depolarization compared to non-conductive substrates. Taken together, these data suggest that blended, conductive scaffolds are stable, supportive of electrical coupling in hPSC-CMs, and promote maturation, which may improve our ability to model cardiac diseases and develop targeted therapies.
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