In Situ Sodium Chloride Cross‐Linked Fish Skin Collagen Scaffolds for Functional Hemostasis Materials

Abstract Current fish collagen hemostasis for wound healing products is commonly obtained by electrospinning or artificial cross‐linking fish collagen fibers which lacks mechanical properties, and biofunctions. Here, a new bio‐active fish skin scaffold (FSS) is shown using in situ cross‐linked scaleless freshwater fish skin adding adipose‐derived stem cells (ASCs)‐produced exosomes for hemostasis and wound healing. The structure, pore size, and the thickness of FSS is studied by swelling test, Fourier‐transform infrared (FT‐IR) spectra, scanning electron microscope (SEM) images, and histological analysis. The biofunctions of the FSS are also tested in vitro and in vivo. FSS keeps two functional layers: The dermis layer collagen forms a sponge like structure after swelling and in situ cross‐linking treatments. The pore size of the FSS is ≈152 ± 23.54 µm, which is suitable for cells growing, angiogenesis and ASCs exosomes accelerate wound healing. The fat‐rich epidermis layer can keep the wound moisty and clean before completely healed. In vitro and in vivo experimental results indicate that FSS+Exosomes enhances rat skin cavity wound healing. In situ sodium chloride cross‐linked FSS+Exosomes provides a new strategy as functional hemostatic dressing scaffold for wound healing.