EZH2型
免疫组织化学
癌症研究
腺癌
生物
组蛋白H3
病理
作者
Takafumi Onishi,Tsuyoshi Takashima,Masako Kurashige,Kenji Ohshima,Eiichi Morii
标识
DOI:10.1016/j.prp.2022.154071
摘要
Enhancer of zeste homolog 2 (EZH2) epigenetically represses gene expression via trimethylation of lysine 27 on histone 3 (H3K27me3). Non-small cell carcinoma (NSCLC) has been reported to show high EZH2 and low H3K27me3 expression compared to normal lung tissues, but there are no studies examining the expression of EZH2 and H3K27me3 simultaneously with immunohistochemistry. In the present study, the expression of EZH2 and H3K27me3 was examined in surgically resected NSCLC. We enrolled 27 cases of squamous cell carcinoma (SCC), 73 cases of Lepidic, 77 of Papillary/Acinar, 51 of Solid, 31 of Micropapillary, and 12 of Mucinous subtypes of adenocarcinoma. First, we examined the expression of EZH2 and H3K27me3 in normal and metaplastic bronchial epithelium adjacent to NSCLC. Normal bronchial epithelium showed EZH2 expression in a limited number of basal cells and H3K27me3 expression in surface differentiated cells with cilia or mucus. In metaplastic bronchial epithelium, the number of EZH2-positive cells increased in multilayered basal cells, and H3K27me3-positive cells were observed in the superficial layer. Then, EZH2 and H3K27me3 expression was analyzed in NSCLC. Abundant EZH2 and rare H3K27me3 expression was detected in SCC, Papillary/Acinar, Solid and Micropapillary subtypes. In Mucinous subtype, EZH2 expression was hardly detected, and H3K27me3 expression was detected in almost all tumor cells. EZH2-expressing and H3K27me3-expressing tumor cells were similarly observed in Lepidic subtype, but double immunofluorescence revealed that EZH2 and H3K27me3 expression pattern was mutually exclusive. No co-expression of EZH2 and H3K27me3 was detected in all examined subtypes. To our knowledge, there have been no reports describing mutually exclusive expression pattern of EZH2 and H3K27me3. • EZH2 catalyzes trimethylation of histone, resulting in the production of H3K27me3, but there is no study examining EZH2 and H3K27me3 expression simultaneously with immunohistochemistry in normal lung and non-small cell lung carcinoma (NSCLC). • The expression level of EZH2 and H3K27me3 was different across subtypes of NSCLS; Solid subtype showed the highest EZH2 expression, Mucinous subtype the highest H3K27me3 expression, and Lepidic subtype the similar EZH2 and H3K27me3 expression. • No co-expression of EZH2 and H3K27me3 was detected, indicating their mutually exclusive expression pattern.
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