生物
基因组编辑
清脆的
Cas9
基因组
壁虎
进化生物学
阿诺里斯
计算生物学
模式生物
遗传学
基因
动物
蜥蜴
作者
Takaya Abe,Mari Kaneko,Hiroshi Kanazawa
标识
DOI:10.1016/j.ydbio.2023.02.005
摘要
Reptiles are important model organisms in developmental and evolutionary biology, but are used less widely than other amniotes such as mouse and chicken. One of the main reasons for this is that has proven difficult to conduct CRISPR/Cas9-mediated genome editing in many reptile species despite the widespread use of this technology in other taxa. Certain features of reptile reproductive systems make it difficult to access one-cell or early-stage zygotes, which represents a key impediment to gene editing techniques. Recently, Rasys and colleagues reported a genome editing method using oocyte microinjection that allowed them to produce genome-edited Anolis lizards. This method opened a new avenue to reverse genetics studies in reptiles. In the present article, we report the development of a related method for genome editing in the Madagascar ground gecko (Paroedura picta), a well-established experimental model, and describe the generation of Tyr and Fgf10 gene-knockout geckos in the F0 generation.
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