鼠李糖乳杆菌
氧化应激
结肠炎
活性氧
殖民地化
微生物学
化学
乳酸菌
生物化学
生物
免疫学
发酵
作者
Siyuan Yuan,Xiaomei Dai,Yuqin Zou,Mingyuan Huang,Xue Yang,Feng Gao
标识
DOI:10.1021/acsmacrolett.5c00295
摘要
Probiotics hold significant promise for treating colitis, but their application remains challenging because of insufficient colonization and survival against colorectal oxidative stress. Herein, engineered probiotics were prepared to enhance probiotics' colonization and scavenge the overexpressed reaction oxygen species (ROS) at the site of colitis. First, primary amino groups on the Lactobacillus rhamnosus (LAB) surface were converted to free thiols by a simple one-step imidoester reaction. Then, we used thiol-initiated ring-opening cascade of dithiolanes to prepare a class of structurally dynamic microgels on the LAB surface (LAB-gel) by reacting alpha-lipoic acid (LA) and sodium thioctate (LANa). Finally, a lipid bilayer was coated on the surface of the LAB-gel to improve the stability of the microgel in gastric juice. Engineered probiotics survived an oxidative stress insult (100 μM H2O2) and were able to effectively colonize the colon. Furthermore, engineered probiotics could significantly uptake by macrophages and scavenge intracellular ROS. Engineered probiotics with enhanced colonization and ROS scavenging activity showed promising therapeutic effects on colitis.
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