软骨细胞
软骨
骨关节炎
细胞
关节软骨
生物
细胞生物学
病理
医学
解剖
遗传学
替代医学
作者
Junjie Wang,Zewen Sun,Cheng‐Hao Yu,Haibo Zhao,Mingyue Yan,Shenjie Sun,Xu Han,Tianrui Wang,Tengbo Yu,Yingze Zhang
标识
DOI:10.1016/j.intimp.2024.111496
摘要
Articular cartilage degeneration is one of the major pathogenic alterations observed in knee osteoarthritis (KOA). Mechanical stress has been verified to contribute to KOA development. To gain insight into the pathogenic mechanism of KOA development, we investigated chondrocyte subsets under different mechanical loading conditions via single-cell RNA sequencing (scRNA-seq). Articular cartilage tissues from both high mechanical loading (named the OATL group) and low mechanical loading (named the OATN group) surfaces were obtained from the proximal tibia of KOA patients, and scRNA-seq was conducted. Chondrocyte subtypes, including a new subset, HTC-C (hypertrophic chondrocytes-C), and their functions, development and interactions among cell subsets were identified. Immunohistochemical staining was also conducted to verify the existence and location of each chondrocyte subset. Furthermore, differentially expressed genes (DEGs) and their functions between regions with high and low mechanical loading were identified. Based on Gene Ontology terms for the DEGs in each cell type, the characteristic of cartilage degeneration in the OATL region was clarified. Mitochondrial dysfunction may be involved in the KOA process in the OATN region.
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