Anti-Aging Effects and Mechanisms of Cod Collagen Peptides (CCPs) in Caenorhabditis elegans

Given the growing interest in natural compounds for promoting healthy aging, this study aimed to investigate the potential of cod collagen peptides (CCPs), a readily available marine resource, to extend lifespan and improve health. Lifespan assays were performed on C. elegans treated with different concentrations of CCPs. Furthermore, various stress resistance assays, including those evaluating oxidative and thermal stress, were conducted. To elucidate the underlying mechanisms, gene expression analysis of key aging-related genes was performed. The results demonstrated that treatment with 25 mg/mL of CCPs extended the lifespan of C. elegans by 13.2%, increased body length and width by 14.8% and 20.6%, respectively, and enhanced head-swing and body-bending frequencies by 66.9% and 80.4%. Lipofuscin content and apoptosis were reduced by 45.9% and 34.1%, respectively. C. elegans treated with 25 mg/mL of CCPs also showed improved stress resistance, a 90.7% increase in glutathione peroxidase (GPX) activity, and a 147.4% increase in glutathione (GSH) content. Transcriptomic analysis showed that CCPs enhanced anti-aging activity by activating the MAPK pathway and inhibiting the IIS pathway, which was associated with protein aggregation. It also reduced lipid synthesis and regulated lipid metabolism through the fat-6 pathway. The results indicated that CCPs could be employed as a valuable ingredient in the food and pharmaceutical fields.