化学
微流控
检出限
线性范围
纳米技术
生物传感器
纳米材料
纳米颗粒
胶体金
色谱法
过氧化氢
银纳米粒子
酶
过氧化物酶
生物化学
材料科学
作者
Kawin Khachornsakkul,Frank J. Rybicki,Sameer Sonkusale
出处
期刊:Talanta
[Elsevier]
日期:2023-08-01
卷期号:260: 124538-124538
被引量:7
标识
DOI:10.1016/j.talanta.2023.124538
摘要
In this study, nanomaterials capable of enzyme-free glucose quantification and colorimetric readout are integrated into a microfluidic paper-based analytical devices (μPADs). Gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs) were utilized as a peroxidase-like nanozyme and a colorimetric probe to achieve glucose monitoring. In this developed device, glucose is oxidized by AuNPs to generate hydrogen peroxide (H2O2), which flows in the paper microchannels toward detection zones. H2O2 then etches the immobilized AgNPs to induce a color change. The intensity of color change is easily monitored using a smartphone application. Following method optimization, we obtained a linear range from 0.50 to 10.0 mmol L−1 (R2 = 0.9921) and a detection limit (LOD) of 340.0 μmol L−1. This falls in the clinically relevant range for glucose monitoring and diabetes diagnosis in humans. In addition, the total analysis time is just 20 min, which is significantly less than the same experiment performed in the solution phase. Also, our method is markedly selective; other substrates do not interfere. The recovery test in human control samples was in the range of 98.47–102.34% and the highest relative standard deviation (RSD) was 3.58%. The enzyme-free approach for glucose sensing is highly desirable for diabetes diagnosis as it replaces the more expensive enzyme with cheaper nanomaterials. Furthermore, since nanomaterials are more environmentally stable compared to enzymes, it has the potential for widespread deployment as point-of-care diagnostics (POC) in resource-limited settings.
科研通智能强力驱动
Strongly Powered by AbleSci AI