Chitosan‐collagen‐fibrinogen uncrosslinked scaffolds possessing skin regeneration and vascularization potential

Abstract Clinical success of regenerative medicine for treating deep‐tissue skin injuries depends on the availability of skin grafts. Though bioengineered constructs are tested clinically, lack of neovascularization provide only superficial healing. Thus constructs, which promotes wound healing and supports vascularization has gained priority in tissue engineering. In this study, chitosan‐collagen‐fibrinogen (CCF) scaffold was fabricated using freeze‐drying method without using any chemical crosslinkers. CCF scaffolds proved cytocompatibility and faster healing in in vitro scratch assay of primary human adult dermal fibroblasts cells with progressively increasing vascular endothelial growth factor‐A and reducing vascular endothelial growth factor receptor 1 expressions. Skin regeneration evaluated on in vivo full thickness wound model confirmed faster remodeling with angiogenic signatures in CCF scaffold‐implanted mice. Histopathological observations corroborated with stereo‐zoom and SS‐optical coherence tomography images of wound sites to prove the maturation of healing‐bed, after 12 days of CCF implantation. Therefore, it is concluded that CCF scaffolds are promising for skin tissue regeneration and demonstrates pro‐angiogenic potential.