生物传感器
纳米材料
纳米技术
检出限
脱氧核酶
异质结
食源性病原体
转导(生物物理学)
化学
材料科学
细菌
光电子学
生物
生物化学
色谱法
单核细胞增生李斯特菌
遗传学
作者
Sadman Sakib,Zijie Zhang,Enas Osman,Farhaan Kanji,Fatemeh Bakhshandeh,Cédric Pattyn,Yingfu Li,Igor Zhitomirsky,Leyla Soleymani
出处
期刊:Nano Today
[Elsevier]
日期:2023-04-01
卷期号:49: 101783-101783
被引量:4
标识
DOI:10.1016/j.nantod.2023.101783
摘要
There is a need for rapid and field-based bacterial detection and identification platforms that do not rely on lengthy growth cultures and lab-based techniques such as mass spectroscopy and polymerase chain reaction. Electrochemical techniques have been developed for culture-free bacterial detection; however, biosensors that deliver a low enough limit-of-detection for direct water pathogen sensing remain elusive. Herein, we report a photoelectrochemical biosensor using photoactive DNAzymes for bacterial identification and photoelectrochemical signal transduction. The signal transduction is based on using DNA as a switch that makes and breaks semiconductor heterostructures between TiO2 nanomaterials for target induced-signal modulation. The developed assay, integrating molecular and photoelectrochemical switching, is able to detect Escherichia coli at a concentration of 18 CFU mL−1 and quantify it over five orders-of-magnitude in lake water without target enrichment, addition of reagents, or sample processing and can specifically identify this organism amongst five similar bacterial pathogens.
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