Delineation of chicken immune markers in the era of omics and multicolor flow cytometry

流式细胞术 免疫系统 生物 计算生物学 免疫学
作者
Sonja Härtle,Kate Sutton,Lonneke Vervelde,Tina Sørensen Dalgaard
出处
期刊:Frontiers in Veterinary Science [Frontiers Media]
卷期号:11
标识
DOI:10.3389/fvets.2024.1385400
摘要

Multiparameter flow cytometry is a routine method in immunological studies incorporated in biomedical, veterinary, agricultural, and wildlife research and routinely used in veterinary clinical laboratories. Its use in the diagnostics of poultry diseases is still limited, but due to the continuous expansion of reagents and cost reductions, this may change in the near future. Although the structure and function of the avian immune system show commonalities with mammals, at the molecular level, there is often low homology across species. The cross-reactivity of mammalian immunological reagents is therefore low, but nevertheless, the list of reagents to study chicken immune cells is increasing. Recent improvement in multicolor antibody panels for chicken cells has resulted in more detailed analysis by flow cytometry and has allowed the discovery of novel leukocyte cell subpopulations. In this article, we present an overview of the reagents and guidance needed to perform multicolor flow cytometry using chicken samples and common pitfalls to avoid.
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